FT-IR

 

Under the FT-IR tab on the MALDI-UP website, we compile tools and resources for microbiological applications of Fourier transform infrared spectroscopy.

FT-IR has been used for the characterization of microorganisms for > 25 years. The applications include identifications at the species level and determination of sero- and biotypes or pathogenicity factors. The direct comparison of spectra also often enables a rapid analysis of contamination routes.

The measuring principle:
FT-IR maps the entirety of the biochemical composition of microorganisms. Molecules are stimulated to move (vibrate, rotate) by infrared rays (heat radiation). Certain bonds in chemical functional groups absorb radiation energy. The absorption of molecular bonds in biomolecules such as fats, proteins or ribonucleic acids at different wavelengths of infrared light leads to a characteristic overall spectrum that reflects the current metabolic status or phenotype of the cells. By standardizing the cultivation conditions, reproducible spectra can be generated from the isolates, which - like a fingerprint - represent a typical pattern for the respective isolate. Using artificial neural networks, the technique shows a very good resolution in taxonomic areas far below the species level.

 

Validation

Using the principles of the Guidelines for validating species identifications using MALDI-TOF-MS (BVL, 28 Oct 2022)

 

Serogroups of Listeria monocytogenes

 

Serogroups of Salmonella

 

Publications

Note: The following list is only a selection of available literature on the subject of FT-IR.

 

Cordovana M, Dogan E, Kostrzewa M, Bohnert JA, Heiden SE, Eer E, Becker K, Schaufler K, Idelevic I
Fourier-transform infrared spectroscopy reliably differentiates capsular types in hypervirulent and classical Klebsiella pneumoniae (Poster)
35. ECCMID - European Congress of Clinical Microbiology and Infectious Diseases 27.-30. 04.2024 Barcelona
https://maldi-up.ua-bw.de/docs/ECCMID2024_IRB ...

 

Cordovana M, Hans JB, Pranada AB
Application of Fourier transform infrared spectroscopy for a neonatal Kluyvera ascorbata clinical outbreak (Poster)
35. ECCMID - European Congress of Clinical Microbiology and Infectious Diseases 27.-30. 04.2024 Barcelona
https://maldi-up.ua-bw.de/docs/ECCMID2024_IRB ...

 

Cordovana M, Mauder N, Pranada AB, Pankok F, Loderstaedt U, Scheithauer S, Dekker D, Zautner AE, Geißdörfer W, Overhoff J, Werner M, Wille A, Frickmann H
Evaluation of FT-IR spectroscopy and machine learning for the discrimination of Escherichia coli and Shigella spp (Poster)
35. ECCMID - European Congress of Clinical Microbiology and Infectious Diseases 27.-30. 04.2024 Barcelona
https://maldi-up.ua-bw.de/docs/ECCMID2024_IRB ...

 

Dinkelacker AG, Vogt S, Oberhettinger P, Mauder N, Rau J, Kostrzewa M, Rossen JWA, Autenrith IB, Peter S, Liese J
Typing and species identification of clinical Klebsiella isolates by Fourier-transform infrared (FTIR) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (Journal)
Journal of Clinical Microbiology 56 e00843-18
https://jcm.asm.org/content/56/11/e00843-18

 

Drigo I, Guolo A, Rizzardi A, Cordovana M, Palazzolo L, Garbuio M, Cordiolo B, Zandona L, Bano L
Machine learning-based typing of Clostridium botulinum by FT-IR spectroscopy: preliminary results (Poster)
35. ECCMID - European Congress of Clinical Microbiology and Infectious Diseases 27.-30. 04.2024 Barcelona
https://maldi-up.ua-bw.de/docs/ECCMID2024_IRB ...

 

Eisenberg T, Mauder N, Contzen M, Rau J, Ewers C, Schlez K, Althoff G, Schauerte N, Geiger C, Margos G, Konrad R, Sing A
Outbreak with clonally related isolates of Corynebacterium ulcerans in a group of water rats (Journal)
BMC Microbiology 15 42
https://bmcmicrobiol.biomedcentral.com/articl ...

 

Kuhm AE, Suter D, Felleisen R, Rau J
Identification of Yersinia enterocolitica at the species and subspecies levels by Fourier transform infrared spectroscopy (Journal)
Applied and Environmental Microbiology 75 5809-5813
https://aem.asm.org/content/75/18/5809

 

Manzulli V, Cordovana M, Serrecchia L, Rondinone V, Pace L, Farina D, Cipolletta D, Caruso M, Fraccalvieri R, Difato LM, Vetritto V, Galante D
Application of Fourier transform infrared spectroscopy to discriminate B. anthracis from B. cereus sensu stricto (Poster)
35. ECCMID - European Congress of Clinical Microbiology and Infectious Diseases 27.-30. 04.2024 Barcelona
https://maldi-up.ua-bw.de/docs/ECCMID2024_IRB ...

 

Martel A, Boyen F, Rau J, Eisenberg T, Sing A, Berger A, Chiers K, Van Praet S, Verbanck S, Vervaeke M, Pasmans F
Widespread Disease in Hedgehogs (Erinaceus europaeus) caused by toxigenic Corynebacterium ulcerans (Journal)
Emerging Infectious Diseases 27 2686-2690
https://doi.org/10.3201/eid2710.203335

 

Mauder N, Cordovana M, Burgwinkel T, del Pino RA, Xanthopoulou K, Wendel AF, Higgins PG
Differentiation of Pseudomonas aeruginosa clinical outbreaks by Fourier transform infrared spectroscopy (Poster)
35. ECCMID - European Congress of Clinical Microbiology and Infectious Diseases 27.-30. 04.2024 Barcelona
https://maldi-up.ua-bw.de/docs/ECCMID2024_Pse ...

 

Mauder N, Cordovana M, Curtoni A, Pastrone L, Genco M, Sorba M, Fedele S, Rosati F, Caroppo MS, Camaggi A, Costa C, Kostrzewa M
Application of Fourier-transform infrared spectroscopy for the differentiation of Mycobacteroides abscessus complex subspecies (Poster)
35. ECCMID - European Congress of Clinical Microbiology and Infectious Diseases 27.-30. 04.2024 Barcelona
https://maldi-up.ua-bw.de/docs/ECCMID2024_IRB ...

 

Mauder N, Rau J
Identification of Salmonella Enterica Serovar Enteritidis Vaccine Strains by Fourier Transform Infrared Spectroscopy (Journal)
Aspects of Food Control and Animal Health 01 
https://www.cvuas.de/ejournal/issue201401.asp

 

Nagib S, Rau J, Sammra O, Lämmler C, Schlez K, Zschöck M, Pfenger-Berninghoff E, Klein G, Abdulmawjood A
Identification of Trueperella pyogenes Isolated from Bovine Mastitis by Fourier Transform Infrared Spectroscopy (Journal)
PLOS One 9(8) e104654
https://doi.org/10.1371/journal.pone.0104654

 

Nardy E, Contzen M, Rau J
Aeromonas oder Deefgea? Sichere Identifizierung durch MALDI-TOF MS und FT-IR (Poster)
16. EAFP-Gemeinschaftstagung der Deutschen, Österreichischen und Schweizer Sektion  05.-07.10.2016 Graz
https://www.ua-bw.de/pub/beitrag.asp?subid=&T ...

 

Oberreuter H, Dyk M, Rau J
Validated differentiation of Listeria monocytogenes serogroups by FTIR spectroscopy using an Artificial Neural Network based classifier in an accredited official food control laboratory (Journal)
Clinical Spectroscopy 5 100030
https://doi.org/10.1016/j.clispe.2023.100030

 

Soike D, Schulze C, Kutzer P, Ewert B, van der Grinten E, Schliephake A, Ewers C, Rau J
Akute Pasteurellose bei Damwild, Rindern und Schweinen in einer Region im Osten Deutschlands (Journal)
Berliner und Münchner Tierärztliche Wochenschrift 125 122-128
https://www.vetline.de/akute-pasteurellose-be ...

 

Stamm I, Hailer M, Depner B, Kopp PA, Rau J
Yersinia enterocolitica in Diagnostic Fecal Samples from European Dogs and Cats: Identification by Fourier Transform Infrared Spectroscopy and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (Journal)
Journal of Clinical Microbiology 51 887-893
https://doi.org/10.1128/JCM.02506-12

 

Wortberg F, Nardy E, Contzen M, Rau J
Identification of Yersinia ruckeri from diseased salmonid fish by Fourier Transform Infrared Spectroscopy (Journal)
Journal of Fisch Disease 35 1-10
https://doi.org/10.1111/j.1365-2761.2011.0131 ...