Project Log 2026

 

MALDI-Kurs zur Artbestimmung am CVUA Stuttgart

Wir freuen uns auf unseren 6. PRAXISKURS für Anwender der MALDI-TOF MS zur Artbestimmung von
Tierischem, Pflanzen & Mikroorganismen.
Aufbau und Erweiterung von Datenbanken, Validierung

In deutscher Sprache
In Präsenz – mit Laborpräsentationen
11.05.2026 (14:00 Uhr) – 13.05.2026 (12:30 Uhr)
Am CVUA Stuttgart, FELLBACH, Baden-Württemberg
13 h Schulung mit 10–12 Teilnehmenden
Kostenbeitrag 400 €

Freuen Sie sich auf die Expertinnen und Experten:
Dr. Rebekka Bonke (LHL Gießen),
Tomke Asendorf (CVUA Freiburg),
Christina Deser, Martin Dyk, Olivera Wenninger (CVUA Stuttgart).

Kursleitung: Dr. Jörg Rau

Sie sind interessiert?
Hier gehts zum Flyer.

Anmeldeschluss 20.03.2026
Die Plätze werden nach der Reihenfolge des Anmeldedatums vergeben.

Bei Fragen melden Sie sich gerne bei uns.

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Update of the MALDI-UP catalogue – 9,240 reference entries listed

MALDI-UP – Status 18.02.2026
Total reference entries: 9240 (+204)
Total validation spectra: 11762 (+49)
Total IR-Spectra: 1671 (+1039) from 261 Isolates

We have now included further MSPs for fungi from the EMALiMB 3.0 database (RIKEN, Japan) in the MALDI-UP catalog. Initial references for Mucoromycota and Mortierellomycota were also listed.
Among the parasites from EMALiMB, first msp are listed for Trypanosoma cruzi, the causative agent of Chagas disease, and for Trichomonas vaginalis.
Furthermore 14 new reference entries from the meat (3), and from the plant database (4) are included.

See: our MALDI-UP catalogue

So far, now 51 institutions or sources from 10 countries have participated.

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Reference spectra available for exchange:

  • 4832  bacteria
  • 1725 fungi (+183)
  • 953 meat (+3)
  • 822 fish + mollusca
  • 113 insects and 123 crustacea
  • 26 parasites (+14)
  • 426 plants (+4)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6590 bacteria (+42)
  • 227 fungi
  • 1910 meat (+3)
  • 906 fish + 20 mollusca
  • 99 insects and 509 crustacea
  • 30 parasites
  • 923 plants (+4)
  • 546 food and milk products
  • 2 cell line

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FT-IR:

Additional data for 1039 IR-Biotyper spectra from 167 isolates (serogroups of Salmonella sp.) are listed.

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Response to call #02 for single spectra of Staphylococcus epidermidis with checked species status

Staphylococcus epidermidis on species level – Validation report for MALDI Biotyper shared:
On 15th January, our group made a call for single spectra from confirmed Staphylococcus epidermidis of other institutions. We got support of several MALDI users (e.g. University Göttingen, Germany; LALLF, Mecklenburg-Vorpommern, Germany), with spectra from well described isolates (e.g.from Nißler et al., 2020¸ Nature Communications 11: 5995). These single spectra are now listed in the MALDI-UP catalogue. Thanks a lot for your help!

This validation study for S. epidermidis is performed analogue the criteria from the guidelines (BVL, 2022).
Now, the report for a single laboratory is available at the tools section.

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Update of the MALDI-UP catalogue – More than 9,000 reference entries listed

MALDI-UP – Status 03.02.2026
Total reference entries: 9036 (+471)
Total validation spectra: 11713 (+36)
Total IR-Spectra: 631 from 91 Isolates

We have now included all MSPs for bacteria from the EMALiMB 3.0 database in the MALDI-UP catalog. In addition to the reference spectra from the JCM collection, the GTC isolate pool is now also integrated. The specific naming conventions of these MSPs require some practice when reading reports. We have also noted in the MALDI-UP catalog any typos or taxonomy errors we noticed of the freely available, well-curated Japanese database.
Furthermore, 14 new reference entries from the microbe (4), the meat (2), and from the plant database (8) are listed.

See: our MALDI-UP catalogue

So far, now 51 institutions or sources from 10 countries have participated.

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Reference spectra available for exchange:

  • 4832  bacteria (+459)
  • 1542 fungi
  • 950 meat (+2)
  • 822 fish + mollusca
  • 113 insects and 123 crustacea
  • 26 parasites
  • 422 plants (+8)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6548 bacteria (+24)
  • 227 fungi
  • 1907 meat (+2)
  • 906 fish + 20 mollusca
  • 99 insects and 509 crustacea
  • 30 parasites
  • 919 plants (+10)
  • 546 food and milk products
  • 2 cell line

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FT-IR:

Data for 631 IR-Biotyper spectra from 91 isolates (serogroups of Listeria monocytogenes).

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We have added several new examples for validation reports in the tools section.

Call #02

The call for single spectra of Staphylococcus epidermidis with checked species status is open until 15.02.2026.

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Call for single spectra of Staphylococcus epidermidis with checked species status

initiated by Jörg Rau, 15.01.2026, CVUA Stuttgart (CVUAS)

We are looking for Bruker MALDI system compatible spectra.

Our goal:
Validation of the actual database, on species level according to the Guidelines for validating using MALDI-TOF-MS (BVL) [see Tools/Validation].

Your benefit:

  • Your spectra will be listed in MALDI-UP
  • For exchange, we offer msp from our own CVUAS collection
  • We will publish the validation report(s) on MALDI-UP

If you have any questions, please contact us.

This call is open until 15.02.2026

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Update of the MALDI-UP catalogue
More than 8,565 reference entries listed – First step to share Infrared spectra via MALDI-UP

MALDI-UP – Status 13.01.2026
Total reference entries: 8565 (+298)
Total validation spectra: 11676 (+18)
Total IR-Spectra: 631 from 91 Isolates

The list for reference entries is extended with > 250 reference spectra from the EMALiMB 3.0 database (Ban et al., 2025). The listing on MALDI-UP of the freely available, well-curated Japanese database will continue.
Furthermore, 11 new reference entries from the meat, and 2 msp from the plant database are listed.

See: our MALDI-UP catalogue

So far, now 51 institutions or sources from 10 countries have participated.

FT-IR

As a new component, we have added a list of infrared spectra of microorganisms to the catalog (NEW: IR-Spectra tab). This list now contains data for 631 IR-Biotyper spectra from 91 isolates from the validation study by Oberreuter et al., 2023 for identification of serogroups of Listeria monocytogenes.
The reports of this study are presented within our FTIR section.

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Reference spectra available for exchange:

  • 4371 bacteria (+285)
  • 1542 fungi
  • 948 meat (+11)
  • 822 fish + mollusca
  • 113 insects and 123 crustacea
  • 26 parasites
  • 414 plants (+2)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6523 bacteria (+4)
  • 227 fungi
  • 1905 meat (+11)
  • 906 fish + 20 mollusca
  • 99 insects and 509 crustacea
  • 30 parasites
  • 909 plants (+2)
  • 546 food and milk products
  • 2 cell line

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Project Log 2025

 

Update of the MALDI-UP catalogue
More than 8,250 reference entries listed, including more than 4,000 from microbes

MALDI-UP – Status 08.12.2025
Total reference entries: 8267 (+315)
Total validation spectra: 11659 (+47)

The list for reference entries is extended with > 250 reference spectra from the EMALiMB 3.0 database (Ban et al., 2025). The listing on MALDI-UP of the freely available, well-curated Japanese database will continue. Furthermore, 18 new reference entries from the meat, and 4 msp from the plant database are listed.

See: our MALDI-UP catalogue

So far, now 51 institutions or sources from 10 countries have participated.

Our visit to the Argentine RENAEM conference (November 3–4, 2025), where we presented our experiences with MALDI-UP, was reported on LinkedIn. #MALDI_UP

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Reference spectra available for exchange:

  • 4086 bacteria (+293)
  • 1542 fungi
  • 939 meat (+18)
  • 822 fish + mollusca
  • 113 insects and 123 crustacea
  • 26 parasites
  • 412 plants (+4)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6519 bacteria (+21)
  • 227 fungi
  • 1896 meat (+18)
  • 906 fish + 20 mollusca
  • 99 insects and 509 crustacea
  • 30 parasites
  • 907 plants (+8)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue
List-entries for Streptococcus sp. from the free CMRS database (Lund University, Sweden) is finalized

MALDI-UP – Status 28.10.2025
Total reference entries: 7952 (+206)
Total validation spectra: 11611 (+49)

The list for reference entries is extended with spectra from the Streptococcus bovis-Streptococcus equinus-species complex (Oeberg et al., 2023; 10.1016/j.diagmicrobio.2023.116045; Suppl.). The source of this CMRS-database is also listed in the Literature sheet.
Furthermore, 20 new reference entries from the plant database are listed.

See: our MALDI-UP catalogue

So far, now 51 institutions or sources from 10 countries have participated.

Events:

We look forward to sharing a report on 10 years of MALDI-UP in one week at the 6th International Meeting of the Argentinean Network of Mass Spectrometry in Buenos Aires. MALDI-UP continues to spread around the globe.

We are now trying out LinkedIn and will link posts about MALDI-UP with #MALDI_UP.

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Reference spectra available for exchange:

  • 3793 bacteria (+173)
  • 1542 fungi
  • 921 meat (+13)
  • 822 fish + mollusca
  • 113 insects and 123 crustacea
  • 26 parasites
  • 408 plants (+20)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6497 bacteria
  • 227 fungi
  • 1878 meat (+14)
  • 906 fish + 20 mollusca
  • 99 insects and 509 crustacea
  • 30 parasites
  • 899 plants (+35)
  • 546 food and milk products
  • 2 cell line

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German standard method for water microbiology – Validation protocol for alternative confirmation including MALDI and IR

Now available: DIN 38411-25 : 2025-11 [German standard]

German standard methods for the examination of water, waste water and sludge

  • Microbiological methods (group K)
  • Protocol for the validation of alternative confirmation procedures and for verification of validated alternative confirmation procedures (K25)

In der oben genannten Neuerscheinung werden für die mikrobiologische Wasseruntersuchung spektrometrische und spektroskopische Verfahren, wie MALDI-TOF MS und FT-IR, ausdrücklich eingeschlossen.
In den Anhängen werden mehrere Beispiele gegeben, wie Parameter für die MALDI-TOF MS auch im Einzellabor validiert werden können, um diese bei der Wassermikrobiologie einzusetzen (z. B. Anhang C, E, F).
Der funktionelle Excel-Report für Anhang C „Clostridium perfringens“ ist im MALDI-UP unter Tools zu finden.
Im informativen Anhang G der Norm werden zudem wichtige Hinweise gegeben, wie mit dynamisch sich weiter entwickelnden Datenbanken umgegangen werden kann.

Die genannte Norm kann bei der DIN Media GmbH, 10772 Berlin  (Hausanschrift: Am DIN-Platz, Burggrafenstraße 6, 10787 Berlin), bezogen werden.

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Update of the MALDI-UP catalogue including list-entries for Streptococcus sp. of the free CMRS database, Lund University, Sweden

MALDI-UP – Status 07.10.2025
Total reference entries: 7746 (+148)
Total validation spectra: 11562 (+71)

The list for reference entries is extended with spectra from the Streptococcus bovis-Streptococcus equinus-species complex (Oeberg et al., 2023; 10.1016/j.diagmicrobio.2023.116045; Suppl.). The source of this CMRS-database is listed in the Literature sheet.

Furthermore, the series of new reference entries from the plant database, made in a research semester of a student from the Esslingen university is extended.

See: our MALDI-UP catalogue

So far, now 51 institutions or sources from 10 countries have participated.

On the website of CVUA Stuttgart, an article about the application of IR spectroscopy in Salmonella was shared in German language on October 6, 2025.

We are now trying out LinkedIn and will link posts about MALDI-UP with #MALDI_UP.

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Reference spectra available for exchange:

  • 3620 bacteria (+131)
  • 1542 fungi
  • 906 meat (+1)
  • 822 fish + mollusca
  • 113 insects and 123 crustacea
  • 26 parasites
  • 388 plants (+16)
  • 204 food and milk products
  • 2 cell line

-------------------------------------

Validation spectra:

  • 6497 bacteria (+44)
  • 227 fungi
  • 1862 meat (+1)
  • 906 fish + 20 mollusca
  • 99 insects and 509 crustacea
  • 30 parasites
  • 864 plants (+27)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue with first database-entries from ReNaEM, Argentina

MALDI-UP – Status 10.09.2025
Total reference entries: 7598 (+87)
Total validation spectra: 11491 (+61)

This update includes a first selection of new reference entries for clinical microbes from ANLIS-Malbrán and its National Mass Spectrometry Network (ReNaEM, Argentina), compatible with the Bruker Biotyper system.
MALDI spectra from 50 institutions are now represented in MALDI-UP.
Furthermore, new reference entries from the meat database, made in a LCiP project, and a first new set of spectra for plants, made in a research semester of a student from the Esslingen university have been added.

In the Tools section, we add new Validation Reports for the Corynebacterium diphtheriae species complex, and for Listeria ivanovii, the second pathogenic Listeria spec.

Furthermore, new reference entries from the meat database made in context of a LCiP-project at the CVUA Stuttgart have been added. This will continue until the next update.

See: our MALDI-UP catalogue

So far, 50 institutions or sources from 10 countries have participated.

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Reference spectra available for exchange:

  • 3489 bacteria (+54)
  • 1542 fungi
  • 905 meat (+8)
  • 822 fish + mollusca
  • 113 insects and 123 crustacea (+1)
  • 26 parasites
  • 372 plants (+8)
  • 204 food and milk products
  • 2 cell line

-------------------------------------

Validation spectra:

  • 6453 bacteria (+44)
  • 227 fungi
  • 1861 meat (+8)
  • 906 fish + 20 mollusca
  • 99 insects and 509 crustacea (+1)
  • 30 parasites
  • 838 plants (+8)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue

MALDI-UP – Status 19.08.2025
Total reference entries: 7527 (+87)
Total validation spectra: 11430 (+14)

This update includes a selection of new reference entries for microbes from the MALDI-GUT database, described and shared by Plomp et al., 2024 University of Groningen, The Nederlands. We have limited the listing of metadata to the MSPs whose name syntax is compatible with the Bruker Biotyper system. The source of the MALDI-GUT-database [Plomp et al., 2024] is listed in the Literature sheet.

Furthermore, new reference entries from the meat database made in context of a LCiP-project at the CVUA Stuttgart have been added. This will continue until the next update.

See: our MALDI-UP catalogue

So far, 48 institutions from 9 countries have participated.

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Reference spectra available for exchange:

  • 3435 bacteria (+81)
  • 1542 fungi
  • 897 meat (+5)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 364 plants (+1)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6410 bacteria (+8)
  • 227 fungi
  • 1853 meat (+5)
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 30 parasites
  • 829 plants (+1)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue / Hint on MALDI-BOT

MALDI-UP – Status 25.07.2025
Total reference entries: 7440 (+11)
Total validation spectra: 11416 (+60)

This update includes some new reference entries from the meat database, and additions for microbes.

The list for single spectra is extended with further spectra for microbes (e.g. Staphylococcus hyicus/chromogenes and Francisella connected with Nothdurfter et al., 2025)

See: our MALDI-UP catalogue

So far, 47 institutions from 9 countries have participated.

We would also like to draw your attention to a new content:
In the Tools/Various section, you will find a link to MALDI-BOT, a virtual assistant specializing in interpretation of mass spectrometry results [RENAEM Argentina]. The reference Prieto et al., 2025 can be found in the Literature section.

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Reference spectra available for exchange:

  • 3354 bacteria (+6)
  • 1542 fungi
  • 892 meat (+9)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 363 plants (+2)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6373 bacteria (+30)
  • 227 fungi
  • 1827 meat (+28)
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 30 parasites
  • 828 plants (+2)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue

MALDI-UP – Status 02.07.2025
Total reference entries: 7423 (+15)
Total validation spectra: 11364 (+89)

This update includes new reference entries from the plant database (eg. Aconitum, Digitalis).

The list for single spectra is extended with further Salmonella created in context of Oberreuter et al., 2025, a validation study for serotyping using the IR-Biotyper (Bruker; preprint).

See: our MALDI-UP catalogue

So far, 47 institutions from 9 countries have participated.
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Reference spectra available for exchange:

  • 3348 bacteria
  • 1542 fungi
  • 883 meat (+5)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 361 plants (+11)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6373 bacteria (+63)
  • 227 fungi (+1)
  • 1827 meat (+14)
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 30 parasites
  • 815 plants (+11)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue

MALDI-UP – Status 23.04.2025
Total reference entries: 7408 (+7)
Total validation spectra: 11276 (+56)

This update includes new reference entries from the plant database (eg. Aconitum).

The list for single spectra is extended with further Yersinia pseudotuberculosis created in context of Riede et al., 2025. In addition, the first Salmonella isolates from a validation study for serotyping using the IR-Biotyper (Bruker) are listed (see Oberreuter et al., 2025; preprint).

See: our MALDI-UP catalogue

So far, 47 institutions from 9 countries have participated.
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Reference spectra available for exchange:

  • 3348 bacteria
  • 1542 fungi
  • 878 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 350 plants (+7)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6310 bacteria (+52)
  • 226 fungi
  • 1813 meat
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 815 plants (+4)
  • 546 food and milk products
  • 2 cell line

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New tap “FT-IR” on MALDI-UP

Under the FT-IR tab on the MALDI-UP website, we compile tools and resources for microbiological applications of Fourier transform infrared spectroscopy.

FT-IR has been used for the characterization of microorganisms for > 25 years. The applications include identifications at the species level and determination of sero- and biotypes or pathogenicity factors. The direct comparison of spectra also often enables a rapid analysis of contamination routes.

In microbiological analytical workflows, FT-IR can be used downstream of species determination by MALDI-TOF MS.

We have posted validation reports for serotyping of Listeria monocytogenes, and Salmonella serogroups here. You will also find references to journal articles, preprints and posters.

As with the main topic MALDI-TOF MS, we would like to encourage interested readers to contribute links and hints to own work. These may also include posters or publications from the past, for example.

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Update of the MALDI-UP catalogue

MALDI-UP – Status 05.03.2025
Total reference entries: 7401 (+8)
Total validation spectra: 11219 (+116)

This update includes several new reference entries from the plant database (seeds). The list for single spectra is extended with 14 Listeria ivanovii (EFEx sample preparation), and 93 Yersinia pseudotuberculosis created in context of Riede et al., 2025, a prevalence study in several zoos in Germany.

See: our MALDI-UP catalogue

So far, 47 institutions from 9 countries have participated.
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Reference spectra available for exchange:

  • 3348 bacteria
  • 1542 fungi
  • 878 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 343 plants (+8)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6257 bacteria (+108)
  • 226 fungi
  • 1813 meat
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 811 plants (+8)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue

MALDI-UP – Status 20.01.2025
Total reference entries: 7393 (+11)
Total validation spectra: 11104 (+147)

This update includes several new reference entries from the plant database (Rubus sp., seeds). Two new MSPs for Corynebacterium diphtheriae species complex were added.

See: our MALDI-UP catalogue

So far, 47 institutions from 9 countries have participated.
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Reference spectra available for exchange:

  • 3348 bacteria (+2)
  • 1542 fungi
  • 878 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 335 plants (+9)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6150 bacteria (+138)
  • 226 fungi
  • 1813 meat
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 803 plants (+9)
  • 546 food and milk products
  • 2 cell line

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Project Log 2024

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 18.12.2024
Total reference entries: 7382 (+41)
Total validation spectra: 10957 (+158)

This update includes several new reference entries from the German Consiliary-Laboratory for Vibrio in food (BfR, Berlin) listed in the MALDI UP catalogue. First free available MSPs for Corynebacterium ramonii, a member of the zoonotic C. diphtheriae species complex made by CVUA Stuttgart were added.

Furthermore, the plant database has been expanded by 18 new reference entries for various berries from LGL Oberschleißheim and CVUA Stuttgart.

With this update, more than 6000 individual spectra of bacteria are listed for validation purposes.

See: our MALDI-UP catalogue

So far, 47 institutions from 9 countries have participated.
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Reference spectra available for exchange:

  • 3346 bacteria (+23)
  • 1542 fungi
  • 878 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 326 plants (+18)
  • 204 food and milk products
  • 2 cell line

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Validation spectra:

  • 6014 bacteria (+128)
  • 226 fungi
  • 1813 meat
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 794 plants (+30)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue

MALDI-UP – Status 18.11.2024
Total reference entries: 7341 (+190)
Total validation spectra: 10799 (+65)

This update incorporates several new reference entries from the Japan Collection of Microorganisms (JCM) and of the Institute of Fungal Morphology (IFM) into the MALDI UP catalogue. With this addition, the database now includes over 1500 entries for fungi. The majority of the database entries from EMALIMB ver. 2 are therefore listed in MALDI-UP.

Furthermore, the plant database has been expanded by 20 new reference entries and nearly 40 validation spectra contributed by CVUA Stuttgart, including several entries for various berries.

See: our MALDI-UP catalogue

So far, 47 institutions from 9 countries have participated.
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Reference spectra available for exchange:

  • 3323 bacteria
  • 1542 fungi (+170)
  • 878 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 308 plants (+20)
  • 204 food and milk products
  • 2 cell line

-------------------------------------

Validation spectra:

  • 5885 bacteria (+28)
  • 226 fungi
  • 1813 meat
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 765 plants (+37)
  • 546 food and milk products
  • 2 cell line

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Update of the MALDI-UP catalogue

MALDI-UP – Status 18.10.2024
Total reference entries: 7151 (+151)
Total validation spectra: 10734 (+82)

A further 145 Basidomycota reference entries from the EMALiMB" (Expanded MALDI-TOF MAS Library for MALDI Biotyper® for fungi and bacteria) have been added.

For the first time, reference spectra for bones are included in the meat section.

The Staatliches Gewerbeaufsichtsamt Hildesheim (Germany) starts presenting msp of specific cell lines.

See: our MALDI-UP catalogue

So far, 47 (+1) institutions from 9 countries have participated.
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Reference spectra available for exchange:

  • 3323 bacteria
  • 1372 fungi (+145)
  • 878 meat (+3)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 288 plants
  • 204 food and milk products
  • 2 cell line

-------------------------------------

Validation spectra:

  • 5857 bacteria (+72)
  • 226 fungi
  • 1813 meat (+3)
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 728 plants (+2)
  • 546 food and milk products (+3)
  • 2 cell line

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Update of the MALDI-UP catalogue

With this update, we are achieving one of our 2024-goals for MALDI-UP:
There are already 7000 reference aspects listed in the catalog for bilateral exchange among users!
New content has also been added to the new IR section.

The Overview:
MALDI-UP – Status 19.09.2024
 Total reference entries: 7000 (+330)
Total validation spectra: 10652 (+11)

Further 310 database entries from the 'EMALiMB' (Expanded MALDI-TOF MAS Library for MALDI Biotyper ® for fungi and bacteria) were listed. We finish the Ascomycota entries and add the first 94 Basidiomycota from this comprehensive reference database for fungi.

Some new Vaccinium and Colchicum entries from the plant database of the CVUA Stuttgart were included.

See: our MALDI-UP catalogue

So far, 46 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 3323 bacteria
  • 1227 fungi (+310)
  • 875 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 286 plants (+9)
  • and 204 food and milk products

Validation spectra:

  • 5785 bacteria (+2)
  • 226 fungi
  • 1810 meat
  • 906 fish + 20 mollusca
  • 98 insects and 509 crustacea
  • 729 plants (+9)
  • and 543 food and milk products

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Corynebacterium diphtheriae group – New validation reports available

Corynebacteria, which are important pathogen agents for humans and animals, are summarized in the Corynebacterium (C.) diphtheriae group. This group includes in particular C. diphtheriae (pathogen of classical diphtheria), C. belfantii, C. ulcerans and C. pseudotuberculosis. Since 2020, further species have been described within the group: C. ramonii, C. rouxii, and C. silvaticum. These species are closely related to each other, making reliable species identification difficult. To date, these important new pathogens have not been integrated in common commercial databases. User made MSPs of all members of the C. diphtheriae group are available for exchange in the MALDI-UP catalog now.

Using these new reference entries, the species identification of the C. diphtheriae group in the Bruker Biotyper system can be improved. As evidence, four formal validations from the CVUA Stuttgart for C. diphtheriae, C. ulcerans+ramonii, C. pseudotuberculosis and C. silvaticum are now presented in the tools-section of the MALDI-TOF MS User Platform.

Targets: members of the C. diphtheriae group:

  • C. diphtheriae
  • C. ulcerans+ramonii
  • C. pseudotuberculosis
  • C. silvaticum

Validation for the BRUKER Biotyper database version K, extended by 1826 msp from the MALDI-UP catalogue.

Fully functional Excel reports in German.

Criteria based on the guidelines for MALDI validation in a single laboratory (BVL, 2022).

The work was carried out in support of the Corynebacterium pseudotuberculosis consiliary laboratory (DVG) at the CVUA Stuttgart.

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Update of the MALDI-UP catalogue

MALDI-UP – Status 26.08.2024
Total reference entries: 6670 (+164)
Total validation spectra: 10642 (+17)

Further 155 database entries (Ascomycota) from the 'EMALiMB' (Expanded MALDI-TOF MAS Library for MALDI Biotyper ® for fungi and bacteria) were listed.

Some new meat entries and new Vaccinium myrtilloides entries from CVUA Stuttgart were included.

Moreover, a first reference entry of the recently described species Cornybacterium ramonii (C. diphtheriae group) is added.

See: our MALDI-UP catalogue

So far, 46 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 3323 bacteria (+2)
  • 916 fungi (+155)
  • 875 meat (+4)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 277 plants (+3)
  • and 203 food and milk products

Validation spectra:

  • 5783 bacteria (+4)
  • 226 fungi
  • 1810 meat (+4)
  • 926 fish + 14 mollusca
  • 98 insects and 509 crustacea
  • 717 plants (+9)
  • and 544 food and milk products

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New validation reports in English available

In the tools section you can find:
Supplement to Rau et al. "Reliable delineation of Bacillus cytotoxicus from other members of the Bacillus cereus group by MALDI-TOF MS - an extensive validation study", Food Control, now available in open-access

Target: Bacillus cytotoxicus, a member of the B. cereus group

Validation for BRUKER Biotyper Database Version K

Validation for the BRUKER Biotyper database version K, extended by 1086 msp from the MALDI-UP catalogue

Fully functional Excel reports

Criteria based on the guidelines for MALDI validation in a single laboratory (BVL, 2022)

In addition, a new validation report for Clostridium perfringens for BRUKER Biotyper Database Version K is available [German].

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 09.07.2024
Total reference entries: 6512 (+292)
Total validation spectra: 10626 (+5)

From the freely available Zenodo dataset of the Robert Koch -Institute (RKI), Berlin all reference entries have now been listed in the MALDI-UP catalogue. With further entries from the genera Brucella, Enterobacter and Burkholderia the set is now completed. The Friedrich-Löffler-Institute (FLI), Jena generated a portion of these spectra.

Moreover, from Institute of Veterinary Bacteriology (IVB) Bern, some reference entries of the recently described species Mannheimia indoligenes were added.


So far, 46 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 3320 bacteria (+291)
  • 761 fungi
  • 870 meat (+1)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 274 plants
  • and 203 food and milk products

Validation spectra:

  • 5779 bacteria (+5)
  • 226 fungi
  • 1806 meat
  • 926 fish + 14 mollusca
  • 98 insects and 509 crustacea
  • 708 plants
  • and 544 food and milk products

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Update of the MALDI-UP catalogue

MALDI-UP – Status 10.06.2024
Total reference entries: 6220 (+439)
Total validation spectra: 10628 (+76)

From the Zenodo dataset of the Robert Koch -Institute (RKI), Berlin a set of reference entries of bacteria (among others: Staphylococcus, Enterococcus, Yersinia,...) including msp for Francisella from the Friedrich-Löffler-Institute (FLI), Jena were added.

Further database entries from the 'EMALiMB' (Expanded MALDI-TOF MAS Library for MALDI Biotyper ® for fungi and bacteria) were listed.


So far, 46 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 3029 bacteria (+405)
  • 761 fungi (+34)
  • 870 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 274 plants
  • and 203 food and milk products

Validation spectra:

  • 5774 bacteria (+72)
  • 226 fungi
  • 1806 meat
  • 926 fish + 14 mollusca
  • 98 insects and 509 crustacea
  • 708 plants (+4)
  • and 544 food and milk products

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Update of the MALDI-UP catalogue

MALDI-UP – Status 02.05.2024
Total reference entries: 5781 (+312)
Total validation spectra: 10545 (+79)

More Clostridia from the Clostri-TOF database (see Tools section and Literature) and further reference entries from the 'EMALiMB' (Expanded MALDI-TOF MAS Library for MALDI Biotyper ® for fungi and bacteria) were listed.

Moreover, new reference entries of Actinobacillus from Ghent University, created in connection with the ENOVAT project, were added.

Furthermore, we added some reference spectra of exotic animals.


So far, 46 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 2624 bacteria (+14)
  • 747 fungi (+284)
  • 870 meat (+14)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 274 plants
  • and 203 food and milk products

Validation spectra:

  • 5702 bacteria (+65)
  • 226 fungi
  • 1806 meat (+14)
  • 926 fish + 14 mollusca
  • 98 insects and 509 crustacea
  • 704 plants
  • and 544 food and milk products

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 08.04.2024
Total reference entries: 5489 (+258)
Total validation spectra: 10465 (+76)

Various Clostridia from the Clostri-TOF database were listed. This database is freely available on Zenodo (Asare et al., 2023). Direct links to the article mentioned and the source of the database are also included in the MALDI-UP literature and toolbox.

Moreover, further reference enries from the 'EMALiMB' (Expanded MALDI-TOF MAS Library for MALDI Biotyper ® for fungi and bacteria) were added.

In addition, we received several new validation spectra from Vaccinium from LAVES Braunschweig/Hannover.


So far, 45 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 2610 bacteria (+140)
  • 463 fungi (+117)
  • 856 meat
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 274 plants (+1)
  • and 203 food and milk products

Validation spectra:

  • 5636 bacteria (+25)
  • 226 fungi
  • 1792 meat (+9)
  • 926 fish + 14 mollusca
  • 98 insects and 509 crustacea
  • 704 plants (+51)
  • and 544 food and milk products

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 11.03.2024
Total reference entries: 5231 (+202)
Total validation spectra: 10389 (+21)

From Robert-Koch Institute (RKI) some Vibrio have been added. Moreover, first lines for database entries from EMALiMB (Expanded MALDI-TOF MAS Library for MALDI Biotyper ® ) from different institutions in Japan were added.

The EMALiMB collection of BRUKER msp (fungi and bacteria) is freely available. A direct link is included in the MALDI-UP toolbox. We will include a selection of this metadata for better searchability in the MALDI-UP catalogue.


So far, 44 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 2470 bacteria (+38)
  • 346 fungi (+136)
  • 856 meat (+10)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 273 plants (+4)
  • and 203 food and milk products

Validation spectra:

  • 5611 bacteria
  • 226 fungi
  • 1762 meat (+9)
  • 926 fish + 14 mollusca
  • 98 insects and 509 crustacea
  • 653 plants(+12)
  • and 544 food and milk products

--------------------------------------

 

Response 2 to call #01

Streptococcus equi:

Subspecies assignments of S. equi equi and of S. equi zooepidemicus/ruminatorum using the collection of MSPs and validation spectra from MALDI-UP.

Strangles, a relevant disease of horses, is connected to the subspecies equi of S. equi.
In diagnostics, the differerentiation of. S. equi equi from the similar ssp S. equi zooepidemicus is a need as ssp. zooepidemicus is frequently isolated as commensals also from horses and other sources.

The reliable differentiation of the ssp. S. e. equi is therefore a prerequisite for targeted veterinary measures.

Following the Validation Guidelines for MALDI-TOF MS Identifications in a single laboratory (BVL, 2022), reports for the subspecies differentiation via MALDI-TOF MS for these two groups were compiled. We share this reports on MALDI-UP in the tools section to activate further exchange among MALDI-UP users.

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 29.01.2024
Now more than 10.000 validation spectra and more than 5.000 database entries are listed in our catalogue.
Total reference entries: 5029 (+239)
Total validation spectra: 10368 (+369)

The State Investigation Office Rhineland-Palatine (LUA-Rheinland-Pfalz) has provided new database entries from meat of Cairina moschata and Cervus elaphus. From BfR Berlin some Vibrio entries were completed. From CVUA Stuttgart new database entries mainly of plants have been contributed.
Moreover, further database entries of microorganisms from the Robert-Koch Institute (RKI) have been listed.
So far, 43 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 2432 bacteria (+175)
  • 210 fungi
  • 846 meat (+24)
  • 822 fish + mollusca
  • 112 insects and 123 crustacea
  • 269 plants (+49)
  • and 203 food and milk products

Validation spectra:

  • 5611 bacteria (+114)
  • 224 fungi
  • 1783 meat (+66)
  • 926 fish + mollusca (+7)
  • 98 insects and 509 crustacea
  • 641 plants (+204)
  • and 561 food and milk products

 

Project Log 2023

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 28.11.2023
Total reference entries: 4798 (+141)
Total validation spectra: 9999 (+118)

The State Institute for Chemical and Veterinary Analysis of Food (CVUA) Freiburg has provided many new database entries, mainly from fish. From the state Institute for Chemical and Veterinary Analysis of Food (CVUA) Stuttgart new database entries of plants have been contributed. Moreover, further database entries of microorganisms by the Robert-Koch Institute (RKI) have been added.
So far, 43 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 2257 bacteria (+39)
  • 210 fungi
  • 822 meat
  • 822 fish + mollusca (+73)
  • 112 insects and 123 crustacea (+7)
  • 220 plants (+22)
  • and 220 food and milk products

Validation spectra:

  • 5497 bacteria
  • 224 fungi
  • 1717 meat
  • 919 fish + mollusca (+72)
  • 98 insects and 516 crustacea (+26)
  • 437 plants (+20)
  • and 561 food and milk products

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 27.10.2023
Total reference entries: 4657 (+198)
Total validation spectra: 9881 (+24)

New database entries from NoKo-LLBB have been added. Above all, special Pasteurellaceae. From CVUA Stuttgart new database entries of bird meat have been contributed. Moreover, further database entries of microorganisms by the Robert-Koch Institute (RKI) have been added.
So far, 43 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 2218 bacteria (+188)
  • 210 fungi
  • 822 meat (+10)
  • 748 fish + mollusca
  • 112 insects and 116 crustacea
  • 198 plants
  • and 220 food and milk products

Validation spectra:

  • 5496 bacteria
  • 224 fungi
  • 1717 meat (+24)
  • 847 fish + mollusca
  • 98 insects and 490 crustacea
  • 417 plants
  • and 561 food and milk products

--------------------------------------

 

Response to call #01

Streptococcus equi on species level - Validation report for MALDI Biotyper shared:

The validation study is performed analogue the criteria from the guidelines (BVL, 2022). The report for a single laboratory version v01 is available at the tools section [see Tools/Validation].

On 5th July, our group made a first call for single spectra from confirmed Streptococcus strains of other institutions. We got support of four MALDI users (STUA Aulendorf, Germany; AGES LINZ, Austria; LALLF, Mecklenburg-Vorpommern, Germany; RWTH Aachen, Germany). Especially helpful were the spectra of the comprehensive reference strain collection of the RWTH Aachen. This support also provided evidence of the robustness of the targeted identification.

We are now continuing our effort towards the subspecies. Independently, further veterinary relevant examples of relevant Streptococcus will follow.

Any further contributions from the user community are welcome via MALDI-UP.

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 22.09.2023
Total reference entries: 4459 (+33)
Total validation spectra: 9857 (+213)

A comprehensive set of validation spectra of reference strains of Streptococcus from the Rheinisch-Westfälische Technische Hochschule (RWHT) Aachen was contributed.
Moreover, further database entries of microorganisms by the Robert-Koch Institute (RKI) and from CVUA Stuttgart were added.
So far, 43 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 2030 bacteria (+23)
  • 210 fungi
  • 812 meat (+6)
  • 748 fish + mollusca
  • 112 insects and 116 crustacea
  • 198 plants (+2)
  • and 220 food and milk products

Validation spectra:

  • 5496 bacteria (+207)
  • 224 fungi
  • 1694 meat (+6)
  • 847 fish + mollusca
  • 98 insects and 490 crustacea
  • 417 plants
  • and 561 food and milk products

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[GERMAN] Veranstaltungshinweis

Unter Events/Current Events auf der MALDI-UP Website finden Sie eine neue online Schulung auf Initiative der Fachgruppe AVID Deutschen Veterinärmedizinischen Gesellschaft (DVG).
14.11.2023: DVG-Fachgruppe AVID-Workshop: MALDI-TOF MS – von der Datenbank zu MALDI-UP // online

Rahmeninfos:

  • Uhrzeit: 08:30–13:00 Uhr (MEZ)
  • Sprache: Deutsch
  • Für DVG Mitglieder ist die Teilnahmegebühr reduziert.

Weitere Details und das Formular zur Anmeldung finden Sie unter dem oben angegebenen Link.

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 04.08.2023
Total reference entries: 4426 (+82)
Total validation spectra: 9641 (+28)

Further database entries of microorganisms by the Robert-Koch Institute (RKI) were listed, as available on Zenodo.
Moreover, first database entries were contributed by CVUA Sigmaringen in cooperation with Albstadt-Sigmaringen University.
So far, 43 institutions from 9 countries have participated.

Reference spectra available for exchange:

  • 1927 bacteria (+80)
  • 210 fungi (+2)
  • 806 meat
  • 748 fish + mollusca
  • 112 insects and 116 crustacea
  • 196 plants
  • and 218 food and milk products

Validation spectra:

  • 5286 bacteria (+28)
  • 224 fungi
  • 1688 meat
  • 847 fish + mollusca
  • 98 insects and 490 crustacea
  • 417 plants
  • and 561 food and milk products

--------------------------------------

 

Call for single spectra of Streptococcus equi with checked subspecies status

Call from CVUA Stuttgart (CVUAS); initiated by Jörg Rau:
We are looking for Bruker MALDI system compatible spectra.

Our goal:
Validation of the actual database, on species and subspecies level according to the Guidelines for validating using MALDI-TOF-MS (BVL) [see Tools/Validation]

Your benefit:

  • Your spectra will be listed in MALDI-UP
  • For exchange, we offer msp from our own CVUAS collection
  • We will publish the validation report(s) on MALDI-UP

If you have any questions, please contact us.

This call is open until 15.08.2023

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 04.07.2023
Total reference entries: 4344 (+567)
Total validation spectra: 9613 (+63)

New database entries of fish from Landesamt für Verbraucherschutz und Lebensmittelsicherheit Institut für Fische und Fischereierzeugnisse Cuxhaven (LAVES IFF CUX) and microorganisms by the Robert-Koch Institute (RKI) were added.
Moreover a database entry for Pseudomonas rustica koreensis, as a recently described species, was contributed by Max Rubner-Institut - Bundesforschungsinstitut für Ernährung und Lebensmittel Kulmbach, Institut für Sicherheit und Qualität bei Fleisch (MRI-FL Kulmbach).
So far, 40 institutions from 8 countries have participated.

Reference spectra available for exchange:

  • 1927 bacteria (+307)
  • 208 fungi
  • 806 meat
  • 747 fish + mollusca (+229)
  • 112 insects and 116 crustacea (+10)
  • 196 plants (+10)
  • and 218 food and milk products

Validation spectra:

  • 5258 bacteria (+31)
  • 224 fungi
  • 1688 meat (+24)
  • 847 fish + mollusca
  • 98 insects and 490 crustacea
  • 417 plants (+6)
  • and 561 food and milk products

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 10.05.2023
Total reference entries: 3777 (+122)
Total validation spectra: 9552 (+64)

New database entries from open data publishing platforms of microorganisms by the Robert-Koch Institute (RKI) and entries of fungi and yeast created by the group Oliver Bader from University Medical Center Göttingen were added.
Moreover first validation spectra from STUA Aulendorf – DZ were listed.
So far, 40 institutions from 8 countries have participated.

Reference spectra available for exchange:

  • 1620 bacteria (+90)
  • 208 fungi (+29)
  • 806 meat (+2)
  • 615 fish + mollusca
  • 112 insects and 97 crustacea
  • 186 plants
  • and 218 food and milk products

Validation spectra:

  • 5227 bacteria (+53)
  • 224 fungi
  • 1664 meat (+7)
  • 835 fish
  • 98 insects and 490 crustacea
  • 411 plants
  • and 561 food and milk products

--------------------------------------

 

Update of the MALDI-UP catalogue

MALDI-UP – Status 04.04.2023
Total reference entries: 3655 (+26)
Total validation spectra: 9488 (+98)

New database entries of bacteria contributed by the Robert-Koch Institute (RKI) and CVUA Stuttgart as well as a first set of new single spectra from the recently published MALDI spectra dataset of the RKI (Zenodo v4) were added. The new contributor Institute for fish and fish products Cuxhaven from the Lower Saxony State Office for Consumer Protection and Food Safety (LAVES-IFF), Germany, added their first database entries of fish species.

Reference spectra available for exchange:

  • 1530 bacteria (+17)
  • 179 fungi
  • 804 meat (+5)
  • 615 fish + mollusca (+4)
  • 112 insects and 97 crustacea
  • 185 plants
  • and 218 food and milk products

Validation spectra:

  • 5174 bacteria (+42)
  • 224 fungi
  • 1657 meat (+5)
  • 835 fish
  • 98 insects and 490 crustacea
  • 410 plants
  • and 561 food and milk products (+54)

--------------------------------------

 

The Project Log has moved!

Since ResearchGate discontinued the project logs, we moved it to our very own website of MALDI-UP. The project log will show you updates about new entries in the MALDI-UP catalogue available for exchange as well as news about protocols or events like the MALDI workshop. Moreover we will send the newest log entry via email to those who are pleased to get the news directly into their mail box. You can cancel your subscription at any time.

Interested?
Simply send an email with "MALDI-UP ON" to

The team of MALDI-UP

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When you subscribe to our project log notifications, the data that is necessary for processing and sending them is collected (email address at least, other personally identifiable information). The data is stored and used exclusively by us and for processing this mail service only. The data is not forwarded to third parties and will be deleted by the date of your expressed cancellation to this mail service.

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